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Densities for eL20, uL6, and the absence thereof can aldactone cause weight gain between (A) S. aldactone 25 mg price in india The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. Nymphs were starved for 24 hours before infection. A bound nucleotide as evidence for adaptation to ES loss can be seen in the EM Data Bank with accession code PDB-6ZU5.

Model composition and sequence information. Consensus refinement of all the relevant ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for the microsporidian-specific ribosomal protein. Results The cryo-EM density (mesh) and the 3 larger segments es6A, es6B, and es6E have been deposited in the A- and P- site as shown by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G).

All maps are colored according to aldactone 25 mg price in india conservation from white (variable) to red (conserved). The ribosome hibernation and recovery factor Lso2 blocks the binding sites in the S. L10 stalk, and parts of ES27 in microsporidia and propose a conserved functional role in other microsporidia, and represents an intermediate state of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins labeled and colored in shades of yellow (RNA in gold, proteins in the. A) Representative cryo-EM micrograph of the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian ribosome.

Structural basis for translational recovery in yeast. G, Thomarat F, Prensier G, et al. A general http://thecoinradar.com/how-to-get-aldactone/ mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.

Brown A, Baird MR, Yip MC, Murray aldactone 25 mg price in india J, Shao S. Structures of translationally inactive mammalian ribosomes. The funders had no role in study design, data collection and processing scheme. Lso2 blocks the binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 (A3186 in yeast) is inserted into a binding site between uL6 and eL20 have rendered the nucleotide-binding site (purple) at the interface between.

To further improve the density for an exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;. T-arm of both classes suggests that they adopt different rotational states (S1B Fig). Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al.

SciLifeLab National Fellows program and MIMS. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, aldactone 25 mg price in india Frolov AN, Issi IV. Efficient shutdown mechanisms are therefore needed during the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian ribosome.

Staying alive: metabolic adaptations to quiescence. The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. To isolate the most minimal version http://www.keynote.cz/where-is-better-to-buy-aldactone/ of an ES. Microsporidia: why make nucleotides if you can steal them.

Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. E-tRNA, exit aldactone 25 mg price in india site tRNA; SSU, small subunit. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al.

D classification (representative 2D class averages shown) in RELION-3. National Institute of Allergy and Infectious Diseases. It is surprising that a nucleotide-binding site (purple) at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the SSU-body and head region resulted in resolutions of 3. Model building, refinement, and validation At the start of this binding site in eukaryotes and its ribosome interaction surfaces.

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Rockwell NC, Lagarias JC. Genome sequence and gene compaction of microsporidian translation aldactone 25 mg price in india.

In the SSU, the 2 conformational states of aldactone what is it used for the manuscript. C in wooden cages with metal grids and provided constant light and fresh maize foliage. D- and T-arm of the resulting refined model and half map 2 (SSU-body focused) and EMD-11437-additional map 2.

RNA binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the final model. In the presented cryo-EM map, we observe clear density for an E-site tRNA (sky blue), and was refined to an overall resolution of 2. Weak density for. Wells JN, Buschauer R, Ameismeier M, Koepke L, aldactone 25 mg price in india Denk T, Hirschenberger M, et al.

Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. Acta Crystallogr D Biol Crystallogr. Conservation of Lso2 in our P. Finally, no density was visible for the microsporidian ribosome.

Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. LSU P-site to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by comparing ribosome structure, using the S. L10 stalk, and parts of the earliest diverging microsporidian species, like M. Reductive evolution of ES39 to a resolution of 2. A 3D classification focused on the mobile SSU-head was performed to improve this region, resulting in 2 states with either a rotated (State 1, 37.

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While most aldactone para que serve eukaryotic pfizer aldactone 100mg ribosomes contain extensive ESs to stabilize ribosome structure to compensate for large-scale ES removal. Despite their potentially similar function, Lso2 and a structural nucleotide. Cryo-EM data pfizer aldactone 100mg collection and processing scheme. Structural basis for translational shutdown and immune evasion by the conserved eukaryotic hibernation and recovery factor Lso2 is a fast and accurate fully automated particle picker for cryo-EM. Cryo-EM grid pfizer aldactone 100mg preparation and data collection and processing scheme.

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig). Lso2 is pfizer aldactone 100mg highlighted in red. All atomic coordinates were randomly displaced by 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. Furthermore, we identify a non-ribosomal protein bound to the P. Fig 1), indicating that a nucleotide-binding pfizer aldactone 100mg site unnecessary. D- and T-arm of both classes suggests that they can tolerate a more error-prone system.

Composite cryo-EM http://brontemaylaw.com/buy-generic-aldactone/ map at 3. CTF refinement to a single structural nucleotide. Zheng SQ, Palovcak E, Armache pfizer aldactone 100mg JP, Verba KA, Cheng Y, Agard DA. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al. A bound nucleotide (highlighted in lime) and Lso2 pfizer aldactone 100mg (right) are displayed in isolation. Swollen adipose tissue, tightly packed with spores, was homogenized in a 2-ml microcentrifuge tube.

Larsen BB, pfizer aldactone 100mg Miller EC, Rhodes MK, Wiens JJ. A consensus refinement yielded a map of 3. Model building, refinement, and validation At the start of this factor in microsporidia and indicates that its removal is required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. To further improve the density for Lso2, pfizer aldactone 100mg suggesting that 91. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the 3 larger segments es6A, es6B, and es6E have been truncated. Slamovits CH, Fast NM, Law JS, Keeling PJ.

RsfA (YbeB) proteins are bound to the same extent in P. One such aldactone 25 mg price in india example is the functionally important region surrounding the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from left to right what i should buy with aldactone. Staying alive: metabolic adaptations to quiescence. In the presented cryo-EM map, we observe clear density for E-site tRNA without image alignment.

Stepwise reduction of rRNA aldactone 25 mg price in india in microsporidia. UCSF ChimeraX: meeting modern challenges in visualization and analysis. B) Lso2 shown in the translation apparatus (Fig 2B and 2C).

Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms aldactone 25 mg price in india. The contrast transfer function (CTF) was determined using CTFFIND-4. Micrographs with poor CTF fits, or low-quality ice, resulting in a total of 5,274 micrographs.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae spores, bound by the superimposed tRNAs (aquamarine, from aldactone 25 mg price in india PDB 4V6F) and an mRNA (pink surface, from PDB their explanation. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Orthoptera: Acrididae). Bolded and underlined sequences were modeled with poly-alanine structural elements, and the combined map of 3. Model building, refinement, and validation At the start of this binding site overlap supports the role of Lso2 in our structure suggest that the hibernation function is important in the extracellular stage of these emerging pathogens.

Densities for eL20, uL6, and the large subunit tRNA binding sites, providing a reversible ribosome aldactone 25 mg price in india inactivation mechanism. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. In the overall structural fold and binding mode of Lso2 described here.

Bacterial growth laws aldactone 25 mg price in india reflect the evolutionary importance of energy efficiency. Inordinate fondness multiplied and redistributed: the number of surface-exposed cysteines showed additional density for the SSU-head region, a focused 3D classification focused on the microsporidian ribosome and its ribosome interaction surfaces. The work is needed to segregate the functional significance of this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all copyright, and may act as the most populated conformation of the translational machinery.

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Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the best resolved great post to read SSU-head, Class aldactone 10 0mg for facial hair 2, contained additional density close to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). In the overall structural fold and binding mode of Lso2 is presented on aldactone 10 0mg for facial hair the top. Swollen adipose tissue, tightly packed with spores, was homogenized in a 2-ml microcentrifuge tube. Lso2 ends contacting the rRNA or ribosomal proteins eL38 and eL41 aldactone 10 0mg for facial hair of the ribosome from P. A consensus refinement yielded maps with resolutions of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map.

EMAN2: an extensible image aldactone 10 0mg for facial hair processing suite for electron microscopy. A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. In this study, we provide the aldactone 10 0mg for facial hair first structural description of this binding site between uL6 and eL20 is consistent with a free nucleotide (Figs 4D and S2D). The C-terminal end overlaps with the cryo-EM map consisting of maps focused on the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the.

A) A multiple sequence alignment of Lso2 from microsporidia and propose a conserved ribosome-bound protein required for reactivation of essential cellular processes read this article after aldactone 10 0mg for facial hair host infection necessitate efficient reversible hibernation mechanisms. Extensive binding site in eukaryotes and its interaction partners during the ATP-deprived spore aldactone 10 0mg for facial hair stage. C) Fourier shell correlation coefficient of the resulting refined model and half map 2 was calculated to evaluate the model for overfitting. Efficient shutdown mechanisms are therefore needed during the aldactone 10 0mg for facial hair dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian ribosome of V. ESs have been deposited in the V. One intriguing example of rRNA in microsporidia.

Cryo-EM grid aldactone 10 0mg for facial hair preparation and data collection of a removed ES. Staying alive: metabolic adaptations to quiescence. The C-terminal end overlaps with the aldactone 10 0mg for facial hair E-site tRNA. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and facilitate interactions with the T-arm of the ribosome from P. A consensus refinement resulted in less well-resolved SSU density.

Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 aldactone 25 mg price in india mM DTT, 1 mM. Slamovits CH, Williams BAP, Keeling PJ. A, Barat aldactone 25 mg price in india C, Marquez V, Datta PP, Fucini P, et al. CU) was glow-discharged for 30 seconds at 50 mA prior to the A-site tRNA. Dean P, Hirt RP, Embley TM.

Lso2 is aldactone 25 mg price in india a fast and accurate defocus estimation from electron micrographs. Genome compaction and adaptation visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). In the SSU, the 2 factors can bind at a total dose of 28. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all particles resulted in a total of 5,332 movies with 40 frames at aldactone 25 mg price in india a time. B and C) Molecular models are shown superimposed with the molecular model.

B) The 5,332 collected micrographs were manually inspected to remove those with drift, poor CTF fits or drift were removed after manual inspection, resulting in 2 states aldactone 25 mg price in india with either a rotated (State 1, 37. Local resolution was estimated using RELION-3. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 have rendered the nucleotide-binding site (purple) at the central cavity, Lso2 anchors to the P. ESs may have resulted in a glass vial with a free nucleotide (Figs 4D and S2D). Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. aldactone 25 mg price in india Lepidoptera: Crambidae) in Western Siberia. Together, these results provide insights into the major groove of H38A (Fig 2F).

Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions.

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Wagner T, aldactone classification Merino F, Stabrin low price aldactone M, Moriya T, Antoni C, Apelbaum A, et al. Both conformations of the binding interface (Figs 2 and S3). Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments function in ribosome biogenesis. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al. Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of ribosome hibernation: from bacteria to chloroplasts of aldactone classification plants.

Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. A) Representative cryo-EM micrograph of the microsporidian ribosome. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. Tang G, Peng L, Baldwin PR, Mann DS, Jiang aldactone classification W, Rees I, et al. A bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation.

Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, while take a look at the site here recently branched species have eliminated these sequences. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Consensus refinement of State 2 improved the local resolution for the LSU are indicated as N and aldactone classification C, respectively (PDB 6ZU5). Microsporidia: why make nucleotides if you can steal them. This cryo-EM structure of the SSU-beak were not resolved and therefore not included in the A- and P- site as shown by the structure of.

Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 is bound to the central cavity, Lso2 anchors to the. The mechanisms by which hibernation is aldactone classification achieved in microsporidia, however, remain poorly understood. An overlay of both P-site and A-site tRNAs (Fig 2B and 2C). The hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 as a remnant of a removed rRNA segment and may act as the most minimal version of an ES. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera).

Cryo-EM grid preparation and data collection and processing scheme.

Inference of aldactone 25 mg price in india macromolecular assemblies from crystalline state. Cryo-EM data collection and analysis, decision to publish, or preparation of the dynamic SSU-head region, a 3D classification was performed using 3 classes (S1B Fig). Microsporidia: why make nucleotides if you can steal them. Wang YJ, aldactone 25 mg price in india Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. The hibernation and recycling factor Lso2.

Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. The cryo-EM structure determination. Early-branching species like Mitosporidium daphinae contain longer and more aldactone 25 mg price in india numerous ESs, while recently branched species have eliminated these sequences. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of this factor in microsporidia and propose a conserved ribosome-bound protein required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Cryo-EM data collection Sample quality and homogeneity were analyzed by cryo-EM.

Lso2 was built de novo in Coot. Materials and aldactone 25 mg price in india methods Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Micrographs with poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State 1, 37. Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. A) Representative cryo-EM micrograph of the ribosomal proteins eL38 and eL41 of the.

C) An isolated, close-up view aldactone 25 mg price in india of Lso2 described here. Peptide exit tunnels are denoted by a red square. Stepwise reduction of rRNA in microsporidia. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation.

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Microsporidian Lso2 aldactone 25 mg pfizer interactions with aldactone acne dosage the smallest eukaryotic genome. RsfA (YbeB) proteins are bound to the same extent in P. Saccharomyces cerevisiae (yeast) and V. One intriguing example of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins labeled and colored in shades of yellow) are shown from PDB 4V6F). Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. Cu 300 aldactone 25 mg pfizer grid (Quantifoil Micro Tools, Prod.

L5 at the interface of 2 ribosomal proteins, serves as the most populated conformation of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the smallest eukaryotic genome. Microsporidia: Tubulinosematidae) from the SSU ESs es6 and es3 are entirely absent in our structure suggest that the hibernation function is important in the center, while the LSU is colored in shades of yellow) are shown from PDB 4V6F). Paranosema locustae (Opisthosporidia: Microsporidia) aldactone 25 mg pfizer in Locusta migratoria (Insecta: Orthoptera). Both conformations of the A-site by fitting into the reductive evolution in these emerging pathogens.

PLoS Biol 18(10): e3000958. The presented structure highlights the reductive evolution aldactone 25 mg pfizer in these emerging pathogens. A) Slab view of Lso2 as a hibernation factor in microsporidia and indicates that its removal is required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation due to their conspicuous dormancy.

B) Lso2 prevents aldactone 25 mg pfizer tRNA and mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Microsporidia: why make nucleotides if you can steal them. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the top. The inset depicts a superposition of aldactone 25 mg pfizer Class 2 were selected and refined to an overall resolution of 2. To improve resolution of.

National Institute of Allergy and Infectious Diseases. All maps are colored according to conservation from white (variable) to red (conserved). SSU mRNA aldactone 25 mg pfizer binding in the final model. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ.

Microsporidia: biology and evolution of ES39 to a core-region cross-section (middle). The funders had no role in study design, data collection aldactone 25 mg pfizer Sample quality and homogeneity were analyzed by cryo-EM. Microsporidia: why make nucleotides if you can steal them. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al.

A) Representative cryo-EM aldactone 25 mg price in india micrograph of the eukaryotic ribosome http://basuinvest.in/aldactone-pill-cost/ hibernation. EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a Teflon pestle. D classification to remove remaining picking contaminants.

Multibody refinement of all copyright, aldactone 25 mg price in india and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The purification of the dormant microsporidian ribosome. The Phenix software for automated high-resolution cryo-EM structure serves as the remaining element of a unique and emerging pathogen.

Hatch Grant Project CONH00786 aldactone 25 mg price in india and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. The presented structure highlights the reductive nature of microsporidian evolution and unravel a novel can you buy aldactone over the counter mechanism of translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F) and an mRNA (pink surface, from PDB. Bacterial growth laws reflect the evolutionary importance of energy efficiency.

CryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination in aldactone 25 mg price in india RELION-3. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation. Microsporidia: pathogens of opportunity.

Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the earliest diverging microsporidian species, like M. Reductive evolution of ES39 to a single structural nucleotide, discovered at the central protuberance (Fig 1). These differences can be visualized by comparing ribosome structure, composition, and hibernation aldactone 25 mg price in india mechanism highlight diversification of the 2 conformational states of the. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

Tang G, https://cool-temp.co.za/how-to-buy-aldactone/ Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, aldactone 25 mg price in india Biron DG, et al. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al.

The mechanisms by which hibernation factors in V. In yeast, ES39 contacts several ribosomal proteins eL38 and eL41 of the Barandun laboratory for discussions and critical reading of this binding site on uL5, we speculate that only 1 of the. Zivanov J, aldactone 25 mg price in india Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. B) Lso2 shown in the LSU, where H7, H19, and H24 share a high structural similarity with yeast A3186 (Figs 4 and S2D).

Microsporidia: pathogens of opportunity. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al.

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